DNA replication and the early to late transition in adenovirus infection

Expression of the late genes of adenovirus is only detectable after virus DNA synthesis has occurred. Using a superinfection protocol, we show that replication of the template per se is required for expression of late regions L2--L5 (mapping to the right of position 39) and that the accumulation of early gene products does not suffice. This regulation is probably exerted at the level of transcription rather than by control of processing or selective stabilization of late mRNA or its precursors. The promoter-proximal late gene block L1, however, appears to be subject to processing control. At early times a single member of this gene family (tripartite leader plus coordinates 29--39, encoding the 52,55K polypeptide pair) is expressed, whereas at late times an additional, differently spliced mRNA species is generated from this region (tripartite leader plus coordinates 34--39, encoding polypeptide IIIa).