Related Experiment Videos
Application of CM-Sephadex C-50 for the production of immunoglobulins
A combined precipitation-ion-exchange chromatographic method is described for the production of immunoglobulin G (IgG) from human plasma. The crude IgG fraction was precipitated with polyethylene glycol (PEG) and further purified on CM-Sephadex C-50. PEG and the contaminating proteins were not bound to the ion-exchange resin; they were eluted with the starting buffer. The purified IgG fraction was obtained in the second step by elution of the proteins bound to the column. In addition to column chromatography a batch procedure has been developed that requires relatively simple equipment. IgG of high purity was obtained by both column chromatography and batch procedure.
A combined precipitation-ion-exchange chromatographic method is described for the production of immunoglobulin G (IgG) from human plasma. The crude IgG fraction was precipitated with polyethylene glycol (PEG) and further purified on CM-Sephadex C-50. PEG and the contaminating proteins were not bound to the ion-exchange resin; they were eluted with the starting buffer. The purified IgG fraction was obtained in the second step by elution of the proteins bound to the column. In addition to column chromatography a batch procedure has been developed that requires relatively simple equipment. IgG of high purity was obtained by both column chromatography and batch procedure.