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Analysis of NZB hyperdiploid spleen cells
Journal of Immunology (Baltimore, Md. : 1950)
|January 1, 1981
Summary
Aneuploid cells in NZB mice originate from bone marrow stem cells and are destined to become B cells. These large, clonally derived cells express H-2 and Ia antigens, indicating a specific B cell lineage.
Area of Science:
- Immunology
- Genetics
- Cell Biology
Background:
- NZB mice exhibit characteristic hyperdiploidy, a condition requiring detailed cell type investigation.
- Understanding the cellular origin of aneuploidy is crucial for comprehending immune system abnormalities in these mice.
Purpose of the Study:
- To identify the specific cell types responsible for hyperdiploidy in NZB mice.
- To elucidate the developmental pathway and characteristics of aneuploid cells in NZB mice.
Main Methods:
- Combined flow cytometry and standard cytogenetic analysis to assess DNA content and chromosomal abnormalities.
- Cytotoxic analysis using specific antibodies (anti-Thy 1, Ly-5, NK, H-2, Ia) to characterize cell surface antigens.
- Bone marrow transplantation experiments in irradiated recipients to trace cell lineage.
Main Results:
- Aneuploid cells in NZB mice are large, clonally derived, and do not express T cell or NK cell markers.
- These cells exhibit increased H-2 antigen expression, linked to extra copies of chromosome 17, and express Ia and Ig.
- Bone marrow transplantation studies confirm that aneuploid cells originate from bone marrow stem cells.
Conclusions:
- The aneuploid cell in NZB mice is a bone marrow-derived stem cell.
- These cells are committed to differentiating into the B cell lineage, specifically a subset deficient in CBA/N mice.
- The findings link hyperdiploidy in NZB mice to a specific developmental pathway within the B cell compartment.