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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

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[Combination of lipid gas and thin-layer chromatography in the diagnosis of functional hepatic failure]

M Ia Orgel', B N Bondarenko, A S Sokol

Voprosy Meditsinskoi Khimii

|July 1, 1980

Abstract:

Gas chromatography of total lipids from blood serum exhibits about 40% error in diagnosis of liver functional insufficiency. Combination of gas chromatography with quantitative thin-layer chromatography and subsequent gas chromatographic analysis of the lipid fractions obtained enabled to increase by 25% the efficiency of diagnoses not only of icteric but also of non-icteric forms of liver functional insufficiency. Simultaneous gas- thin-layer chromatographic analysis of total lipids will apparently improve the differential diagnosis of diseases accompanied by liver functional insufficiency; it permits also to study pathogenesis of impairments in lipid metabolism under conditions of liver functional insufficiency and of other pathologies.

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Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...

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In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.

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