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Updated: Aug 14, 2026
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NanoDrop Microvolume Quantitation of Nucleic Acids
Published on: November 23, 2010
[Fluorometric DNA assay at nanogram levels in biological material]
Bollettino Della Societa Italiana Di Biologia Sperimentale
|August 30, 1980
Summary
This study presents a reproducible microfluorimetric DNA determination method. The assay uses 33258 Hoechst dye and is unaffected by common lab reagents or cellular components.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Context:
- Accurate DNA quantification is crucial in molecular biology and diagnostics.
- Existing DNA determination methods can be time-consuming or require complex procedures.
- Microfluorimetric techniques offer potential for rapid and sensitive DNA analysis.
Purpose:
- To develop and validate a simple, rapid, and highly reproducible microfluorimetric method for DNA determination.
- To assess the reliability of the 33258 Hoechst fluorochrome assay for quantifying DNA in various biological samples.
Summary:
- A microfluorimetric method utilizing the 33258 Hoechst fluorochrome was established for DNA determination.
- The method demonstrates high reproducibility when applied to calf thymus DNA and mouse liver chromatin.
- Crucially, standard preparation reagents and subcellular components do not interfere with the fluorescence-based DNA assay.
Impact:
- Provides a robust and efficient tool for DNA quantification in research and clinical settings.
- Simplifies DNA analysis, potentially reducing experimental costs and time.
- Enhances the reliability of DNA measurements in diverse biological matrices.
