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Related Experiment Videos

Screening for IgE-mediated allergy

T G Merrett, C F Pantin, A H Dimond

    Allergy
    |September 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Screening for common allergens using a mixed-allergen Radioallergosorbent Test (RAST) effectively identifies positive results. Further allergen testing is guided by total serum IgE levels and patient history analysis.

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    Area of Science:

    • Immunology
    • Allergy Diagnostics

    Background:

    • Radioallergosorbent Test (RAST) is a common method for diagnosing allergies.
    • Identifying the most prevalent allergens is crucial for efficient allergy testing.

    Purpose of the Study:

    • To evaluate the effectiveness of a mixed-allergen RAST screening approach.
    • To determine the correlation between mixed-allergen RAST results, total IgE levels, and patient history.

    Main Methods:

    • Analysis of 425 patient sera using Phadebas RAST reagents against 19 allergens.
    • Utilized a mixed-allergen RAST technique combining common UK allergens (grass pollen, Dermatophagoides pteronyssinus, cat epithelium) and a representative mould RAST for initial screening.

    Main Results:

    • The most frequent positive RAST results were for grass pollen, Dermatophagoides pteronyssinus, and cat epithelium.

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  • Of 275 RAST-positive sera, 261 were positive using the mixed-allergen RAST.
  • A positive mixed-allergen RAST often correlated with total serum IgE levels >40 U/ml and positive symptom scores.

    • Conclusions:

    • Mixed-allergen RAST screening, combined with mould RAST, can guide further allergen investigations.
    • Negative results from mixed-allergen and mould RAST suggest no significant allergies among the tested panel.
    • Total IgE levels and computer-assisted history analysis aid in interpreting RAST results and managing allergy investigations.