Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pathological specimens of rat temporal bone--comparison between different fixative solution

M Sano1, T Harada, M Sakagami

  • 1Department of Otorhinolaryngology, Osaka Medical Center, Japan.

Medical Journal of Osaka University
|September 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of a universal three-body s-wave resonance in <sup>10</sup>He.

Nature communications·2026
Same author

[Venous Thromboembolic Disease In Women In The Cardiology Department Of The Kati University Hospital].

Le Mali medical·2024
Same author

Mass, Spectroscopy, and Two-Neutron Decay of ^{16}Be.

Physical review letters·2024
Same author

[Epidemiology of Covid-19 and high blood pressure association at Mali'shospital].

Le Mali medical·2023
Same author

Quasifree Neutron Knockout Reaction Reveals a Small s-Orbital Component in the Borromean Nucleus ^{17}B.

Physical review letters·2021
Same author

Surface Localization of the Dineutron in ^{11}Li.

Physical review letters·2021
Same journal

Genotoxic potentials of lifestyles assessed by urinary mutagenicity.

Medical journal of Osaka University·1994
Same journal

Disorder in ultrastructure of basement membrane and mechanical junction in human esophageal cancer.

Medical journal of Osaka University·1994
Same journal

Pancreas cancer-associated antigen (PCAA) in medullary thyroid carcinoma.

Medical journal of Osaka University·1994
Same journal

Electron microscopic observation of bFGF immunoreactivity in the hippocampus.

Medical journal of Osaka University·1994
Same journal

Liver infarction produced by dual vessels occlusion due to trauma and TAE: long follow up using CT, US, and MRI.

Medical journal of Osaka University·1993
Same journal

Comparison of neural network and k-NN classification methods in medical image and voice recognitions.

Medical journal of Osaka University·1993
See all related articles

Heidenhein-SuSa fixative is the superior method for temporal bone fixation, preserving cellular structures without precipitates or detachment. Other fixatives like formalin and Wittmaack's cause artifacts, impacting histological analysis.

Area of Science:

  • Histology
  • Otolaryngology
  • Anatomical Pathology

Background:

  • Proper fixation of temporal bone specimens is crucial for accurate histological examination.
  • Artifacts introduced by fixative solutions can compromise the integrity of delicate cochlear structures.

Purpose of the Study:

  • To compare the efficacy of three different fixative solutions (10% formalin, Wittmaack's, and Heidenhein-SuSa) in preserving temporal bone specimens.
  • To identify fixation-induced artifacts and determine the optimal fixative for histological analysis of the inner ear.

Main Methods:

  • Temporal bone specimens were fixed using 10% formalin, Wittmaack's fixative, and Heidenhein-SuSa fixative.
  • Histological sections were stained with hematoxylin-eosin to evaluate cellular morphology and identify precipitates.

Related Experiment Videos

  • Microscopic examination focused on the cochlea, vestibule, tectorial membrane, and sulcus cells.

    • Main Results:

    • 10% formalin fixation resulted in pinkish precipitates in the scala media, perilymphatic, and endolymphatic spaces, along with cellular fusion in various cochlear structures.
    • Wittmaack's fixative led to the detachment of inner and outer sulcus cells from the basilar membrane and notable Reissner's membrane deformation.
    • Heidenhein-SuSa fixative demonstrated no precipitates, cellular fusion, or cell detachment, indicating superior preservation.

    Conclusions:

    • Heidenhein-SuSa fixative is the most effective method for temporal bone fixation, yielding artifact-free histological preparations.
    • Formalin and Wittmaack's fixatives introduce significant artifacts that can hinder accurate interpretation of inner ear pathology.
    • Optimal fixation is essential for reliable histological studies of the temporal bone and inner ear structures.