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Inhibition of inducible nitric oxide synthase mRNA expression by basic fibroblast growth factor in human microglial
M Colasanti1, T Di Pucchio, T Persichini
1Department of Biology, III University of Rome, Italy.
Neuroscience Letters
|July 28, 1995
Abstract:
The effect of basic fibroblast growth factor (bFGF) on inducible nitric oxide synthase (iNOS) mRNA expression in human cultured ramified microglial cells was investigated. Using RT-PCR and Southern blot analysis, we found that bFGF prevented the iNOS gene expression as induced by LPS/TNF alpha. Also, bFGF dose-dependently inhibited nitrite levels in treated cell supernatants. That the early presence of bFGF during LPS/TNF alpha induction was essential indicates that iNOS gene expression can be transcriptionally regulated.