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Quantitative determination of E- and Z-doxepin and E- and Z-desmethyldoxepin by high-performance liquid
M Adamczyk1, J R Fishpaugh, C Harrington
1Division Organic Chemistry Research, Abbott Laboratories, Abbott Park, Illinois 60064, USA.
Therapeutic Drug Monitoring
|August 1, 1995
Abstract:
A high-performance liquid chromatographic (HPLC) method was developed for the quantitative, simultaneous determination of the following four compounds in serum: E-doxepin, Z-doxepin, E-desmethyldoxepin, and Z-desmethyldoxepin. A 3-microns analytical silica column (6 x 100 mm) was employed with the mobile phase 0.025 M phosphate:acetonitrile:n-nonylamine (80:20:1). This HPLC method allows for the accurate measurement of all four isomeric compounds.