Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydroxy- and non-hydroxy-galactolipids in developing rat CNS

C G De Haas1, M Lopes-Cardozo

  • 1Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Contribution of acetoacetate to the synthesis of cholesterol and fatty acids in regions of developing rat brain in vivo.

Neurochemistry international·2010
Same author

Substrate utilization for energy production and lipid synthesis in oligodendrocyte-enriched cultures prepared from rat brain.

Neurochemistry international·2010
Same author

Relationship between the pentose-phosphate pathway and the de novo synthesis of fatty acids and cholesterol in oligodendrocyte-enriched glial cultures.

Neurochemistry international·2010
Same author

Ketone-body utilization and lipid synthesis by developing rat brain-a comparison between in vivo and in vitro experiments.

Neurochemistry international·2010
Same author

Loading-induced changes in synovial fluid affect cartilage metabolism.

British journal of rheumatology·1998
Same author

A microtiter plate assay for the determination of uronic acids.

Analytical biochemistry·1998
Same journal

Scaphocephaly as a Novel Phenotypic Feature of WDR83OS-Related Neurodevelopmental Disorder: A Case Report With a Novel Variant and Literature Review.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
Same journal

Sales-Training-Inspired Optimization for Deep High-Order Principal Network in Autism Spectrum Disorder Classification.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
Same journal

Turkish Validity and Reliability Study of the Executive Functioning Scale and the Daily Living Skills Scale in Children and Adolescents.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
Same journal

Neuropsychiatric Features, ADHD and Cognitive Disengagement Syndrome in Children and Adolescents With Celiac Disease: A Controlled Study.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
Same journal

The Bright Side of the Spectrum: Self-Limited Infantile Epilepsy-Related SCN8A Gof Variant.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
Same journal

Domain-Specific Cognitive Vulnerabilities in ADHD: The Impact of Comorbid Specific Learning Disorder on WISC-IV Profiles.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2026
See all related articles

Rat spinal cord myelin accumulates galactolipids, including galactosylceramide (GalCer) and sulfated galactosylceramide (SGalCer), during development. These lipids show distinct accumulation patterns and fatty acid profiles in spinal cord versus cerebral tissue, indicating region-specific oligodendrocyte metabolism.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Developmental Biology

Background:

  • Myelin, the insulating sheath around nerve fibers, is crucial for efficient neural signal transmission.
  • Galactolipids, such as galactosylceramide (GalCer) and sulfated galactosylceramide (SGalCer), are key components of mammalian myelin.
  • Understanding the developmental dynamics of myelin lipids is essential for comprehending central nervous system maturation.

Purpose of the Study:

  • To investigate the developmental accumulation patterns of various galactolipids in rat spinal cord myelin.
  • To compare the composition and developmental trends of galactolipids in spinal cord versus cerebral tissue.
  • To elucidate the role of galactolipid metabolism in myelin development within different central nervous system regions.

Main Methods:

Related Experiment Videos

  • High-performance liquid chromatography (HPLC) was used to analyze galactolipid species in rat spinal cord and cerebral tissues from postnatal weeks 1 to 24.
  • Quantification of major galactolipids, galactosylceramide (GalCer) and sulfated galactosylceramide (SGalCer), and galactoglycerolipids was performed.
  • Analysis of the non-hydroxy fatty acid (NFA) to hydroxy fatty acid (HFA) ratio in galactolipid species was conducted.

Main Results:

  • GalCer and SGalCer levels increased linearly in rat spinal cord during the first two postnatal months, reaching plateau levels approximately 4-fold and 2.5-fold higher than in cerebral tissue, respectively.
  • Galactoglycerolipid accumulation preceded galactosphingolipids and decreased with CNS maturation, comprising a smaller fraction of total galactolipids.
  • Distinct differences in the hydroxy-species contribution to GalCer and SGalCer were observed between spinal cord and cerebrum, suggesting region-specific lipid metabolism.

Conclusions:

  • Rat spinal cord exhibits distinct developmental programs for galactolipid metabolism compared to cerebral tissue, particularly in oligodendrocytes responsible for myelin formation.
  • The accumulation and composition of myelin galactolipids, including GalCer and SGalCer, are differentially regulated in high-myelinated (spinal cord) versus low-myelinated (cerebral) areas of the CNS.
  • These findings highlight the complex, region-specific nature of myelin lipid synthesis and its role in CNS development.