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Didemnin B induces cell death by apoptosis: the fastest induction of apoptosis ever described
D R Grubb1, E J Wolvetang, A Lawen
1Department of Biochemistry and Molecular Biology, Monash University, Clayton (Melbourne) Vic, Australia.
Biochemical and Biophysical Research Communications
|October 24, 1995
Abstract:
Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of apoptosis are seen when HL-60 cells are pretreated with 1mM Zn2+ immediately before treatment with didemnin B.