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Mitogenic response to TGF-beta in 3T3-F442A cells
D I Jeoung1, B Tang, M Sonenberg
1Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Biochemical and Biophysical Research Communications
|November 22, 1995
Abstract:
In 3T3-F442A cells, TGF-beta caused cellular proliferation in a time and dose-dependent manner. TGF-beta induced cyclin D1 and cdk2 proteins in 3T3-F442A cells. The mitogenic effect of TGF-beta was specific in nature. The antimitogenic agent, hGH, inhibited the mitogenic effect of TGF-beta and was associated with inhibition of cyclin D1 expression. The protein kinase c inhibitor, staurosporine, inhibited the mitogenic effect of TGF-beta. Taken together, these results suggest that TGF-beta affects expression levels of cell cycle-regulated proteins and its mitogenic effect is mediated through protein kinase C in 3T3-F442A cells.