[Effect of tetramethylpyrazine on lymphocytes proliferation response of murine splenocytes]

The effect of tetramethylpyrazine (TMP) on lymphocytes proliferation response (LPR) detected by 3H-TdR incorporation into murine monocytes. The results showed that TTMP at the concentration of 100-1000 micrograms/ml inhibited LPR in normal mice and B14-melanoma bearing mice in vitro the effect was dose-dependent. It was also found TTMP could stimulate LPR to Con A at the concentration of 1 micron/ml in normal mice, and at the concentration of 100-1000 micrograms/ml in B16-melanoma bearing mice. But TTMP inhibited LPR to Con A at the concentration of 100-1000 micrograms/ml in normal mice. The restoration of LPR to PHA and increasing 3H-TdR incorporation into splenocytes were found in vivo in B16-melanoma bearing mice after treatment with TTMP. The data indicates that TTMP has an immune modulation.