Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro testing of antimicrobial agents

G L Woods1

  • 1University of Texas Medical Branch, Galveston, USA.

Infectious Disease Clinics of North America
|September 1, 1995
PubMed
Summary

Antimicrobial susceptibility testing for inhibition is standardized and guides therapy. However, the clinical value of bactericidal testing and synergism assays requires further research and standardization for reliable interpretation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efforts of the United States' National Marrow Donor Program and Registry to improve utilization and representation of minority donors.

Transfusion medicine (Oxford, England)·2008
Same author

Ertapenem versus cefepime for initial empirical treatment of pneumonia acquired in skilled-care facilities or in hospitals outside the intensive care unit.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2006
Same author

Antimicrobial susceptibility patterns in Pseudomonas aeruginosa: data from a multicenter Intensive Care Unit Surveillance Study (ISS) in the United States.

Journal of chemotherapy (Florence, Italy)·2004
Same author

Management of complicated appendicitis and comparison of outcome with other primary sites of intra-abdominal infection: results of a trial comparing ertapenem and piperacillin-tazobactam.

Journal of chemotherapy (Florence, Italy)·2004
Same author

A rapid immunochromatographic assay for hepatitis B virus screening.

Journal of viral hepatitis·2003
Same author

Expression of the uterine Mx protein in cyclic and pregnant cows, gilts, and mares.

Journal of animal science·2003

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Antimicrobial Stewardship

Background:

  • Antimicrobial susceptibility testing (AST) is crucial for guiding effective and cost-efficient patient therapy.
  • Standardized methods exist for determining antimicrobial inhibitory activity, with established guidelines for quality control and reporting.
  • The interpretation and reporting of AST results require collaboration among clinical microbiologists, hospital committees, and infectious disease clinicians.

Purpose of the Study:

  • To review the current status and clinical utility of antimicrobial susceptibility testing methods.
  • To highlight the need for further research into the clinical value of bactericidal testing and antimicrobial synergism assays.
  • To emphasize the importance of standardization in antimicrobial testing methodologies.

Main Methods:

  • Review of existing guidelines and literature on antimicrobial susceptibility testing.
  • Analysis of standardized methods for determining inhibitory and bactericidal activity.
  • Discussion of challenges in standardizing and interpreting tests for antimicrobial synergism and antagonism.

Main Results:

  • Standardized methods for determining antimicrobial inhibitory activity are well-defined and clinically useful.
  • The clinical value of bactericidal testing is poorly defined, necessitating further clinical trials.
  • Standardization of methods for determining antimicrobial synergism/antagonism is lacking, hindering interpretation and comparison of results.

Conclusions:

  • Standardized inhibitory susceptibility testing effectively guides antimicrobial therapy.
  • Further research, including prospective clinical trials, is essential to define the clinical utility of bactericidal testing.
  • Lack of standardization in synergism testing limits its clinical application and comparability across institutions.

Related Experiment Videos