Related Experiment Videos
Determination of sulphonamides in human urine by azo dye precolumn derivatization and micellar liquid chromatography
E F Simó-Alfonso1, G Ramis-Ramos, M C García-Alvarez-Coque
1Departament de Química Analítica, Facultat de Química, Universitat de València, Spain.
Journal of Chromatography. B, Biomedical Applications
|August 4, 1995
Abstract:
A high-performance liquid chromatographic method for the determination of sulphonamides in urine is reported. The drugs (sulphadiazine, sulphaguanidine, sulphamethizole, sulphamethoxazole, and sulphathiazole) were diazotized with nitrite and coupled with N-(1-naphthyl)ethylenediamine dihydrochloride in a sodium dodecyl sulphate (SDS) micellar medium. Separation of the sulphonamide azo dyes was performed on a C18 column with a 0.05 M SDS-2.4% pentanol mobile phase, which permitted the direct injection of the urine samples. The limits of detection were in the 0.1-0.3 micrograms/ml range.