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Gene-targeted mice in leukocyte adhesion research
1Department of Biomedical Engineering, University of Virginia Medical School, Charlottesville 22908, USA.
Microcirculation (New York, N.Y. : 1994)
|August 1, 1995
Summary
Gene-targeted mice lacking specific adhesion molecules reveal their roles in leukocyte trafficking during inflammation. Microcirculatory studies in these models enhance understanding of leukocyte recruitment in health and disease.
Area of Science:
- Immunology
- Cell Biology
- Physiology
Background:
- Leukocyte trafficking is crucial for physiological and inflammatory responses.
- Adhesion molecules on leukocytes and endothelial cells mediate this process.
- Four main families of adhesion molecules exist: integrins, immunoglobulin-like molecules, selectins, and selectin ligands.
Purpose of the Study:
- To review insights gained from studying adhesion molecule-deficient mice.
- To emphasize the role of microcirculatory studies in understanding leukocyte trafficking.
- To clarify molecular mechanisms of leukocyte trafficking in health and disease.
Main Methods:
- Utilizing gene-targeting technology to create mutant mice lacking specific adhesion molecules.
- Employing microcirculatory studies to observe leukocyte recruitment in vivo.
- Investigating mice with combined adhesion molecule deficiencies.
Main Results:
- Adhesion molecule-deficient mice models have been instrumental in studying inflammation.
- Microcirculatory studies are vital for understanding leukocyte recruitment steps.
- These models elucidate the interplay between different adhesion receptors.
Conclusions:
- Adhesion molecule-deficient mice provide critical in vivo models for inflammation research.
- Microcirculatory studies are indispensable for dissecting leukocyte trafficking mechanisms.
- Further research with novel gene targets and combined deficiencies will advance understanding of leukocyte behavior.
