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Related Experiment Videos

How do volatile anesthetics inhibit Ca(2+)-ATPases?

M M Lopez1, D Kosk-Kosicka

  • 1Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.

The Journal of Biological Chemistry
|November 24, 1995
PubMed
Summary
This summary is machine-generated.

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Volatile anesthetics inhibit calcium pumps (Ca(2+)-ATPase) by altering enzyme shape. This disruption affects calcium transport, crucial for cell function.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Cell Biology

Background:

  • Volatile anesthetics are used clinically to induce anesthesia.
  • These anesthetics selectively inhibit the plasma membrane Ca(2+)-transport ATPase (Ca(2+)-ATPase) at clinical concentrations.
  • The precise mechanism of this inhibition is not fully understood.

Purpose of the Study:

  • To investigate the mechanism by which volatile anesthetics inhibit purified erythrocyte Ca(2+)-ATPase.
  • To elucidate how anesthetics affect the enzyme's conformation and Ca(2+)-dependent activation.

Main Methods:

  • Purified erythrocyte Ca(2+)-ATPase was used.
  • Fluorescence spectroscopy measured changes in intrinsic tryptophan and extrinsic probe environments.
  • Ca(2+)-dependent enzyme activity was assessed.

Related Experiment Videos

Main Results:

  • Anesthetic-induced inhibition correlated with the loss of Ca(2+)-induced conformational changes.
  • Anesthetics significantly altered enzyme conformation, as indicated by tryptophan fluorescence.
  • Similar conformational changes were observed in sarcoplasmic reticulum Ca(2+)-ATPase.

Conclusions:

  • Volatile anesthetics inhibit Ca(2+)-ATPase by binding to nonpolar sites within the protein interior.
  • This binding perturbs enzyme conformation and function, impacting calcium transport.
  • This interaction exemplifies a general mechanism of small molecule binding to internal protein sites.