Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mast cell tryptase levels in normal canine tissues

A D Myles1, R E Halliwell, B Ballauf

  • 1Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.

Veterinary Immunology and Immunopathology
|June 1, 1995
PubMed
Summary

Researchers developed a new enzyme-linked immunosorbent assay (ELISA) to measure canine tryptase in tissues. Canine gut tissue showed the highest tryptase levels, suggesting a significant role for this enzyme in the gut.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Picrotoxin-Barbiturates Antagonism in Decorticated Animals.

The Yale journal of biology and medicine·2011
Same author

Veterinary education: time to abandon the cult of coverage.

The Canadian veterinary journal = La revue veterinaire canadienne·2007
Same author

Serum antibodies to Malassezia yeasts in canine atopic dermatitis.

Veterinary dermatology·2002
Same author

Local lung responses following local lung challenge with recombinant lungworm antigen in systemically sensitized sheep.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2001
Same author

The ACVD task force on canine atopic dermatitis: forewords and lexicon.

Veterinary immunology and immunopathology·2001
Same author

The ACVD task force on canine atopic dermatitis (III): the role of antibodies in canine atopic dermatitis.

Veterinary immunology and immunopathology·2001

Area of Science:

  • Veterinary Medicine
  • Biochemistry
  • Immunology

Background:

  • Canine tryptase is an enzyme found in mast cells.
  • Understanding tryptase distribution is crucial for diagnosing canine diseases.
  • Existing methods for tryptase quantification are limited.

Purpose of the Study:

  • To develop and validate a competition enzyme-linked immunosorbent assay (ELISA) for quantifying canine tryptase.
  • To determine the tissue distribution of canine tryptase.
  • To assess the potential role of this assay in veterinary diagnostics.

Main Methods:

  • A competition enzyme-linked immunosorbent assay (ELISA) was developed using rabbit anti-tryptase antibodies.
  • The assay quantifies tryptase in tissue extracts within a range of 40-5000 ng ml-1.

Related Experiment Videos

  • Tissues analyzed included skin, gut, liver, and lung from dogs.

    • Main Results:

    • The developed ELISA assay demonstrated reliable quantification of canine tryptase.
    • Canine gut tissue exhibited the highest concentration of tryptase per milligram of wet weight compared to other tissues.
    • Skin, liver, and lung tissues also contained measurable levels of canine tryptase.

    Conclusions:

    • The novel ELISA assay is effective for measuring canine tryptase levels in various tissues.
    • The high concentration of tryptase in canine gut suggests a potentially significant functional role at this site.
    • This assay can be a valuable tool for investigating mast cell involvement in canine disease states.