Stability of 2-chloro-2'-deoxyadenosine at various pH and temperature

In this paper data concerning the stability of 2-chloro-2'-deoxyadenosine (2-CdA) solutions at various pH and temperature conditions are presented. Decomposition of 2-CdA was measured as content of its hydrolysis product--2-chloroadenine, using the HPLC method. We found that 2-CdA is stable at basic and neutral pH and temperatures between 37 and 80 degrees C. At acidic pH, decomposition markedly increased with time, even in physiological temperatures. At pH 2 and 37 degrees, after 6 h, only 13% of 2-CdA remained in solution. At pH 1--after 2 h of heating to 37 degrees 2-CdA content was as low as 2%. Calculated half time T 1/2 was equal to 1.6 h (pH 2, temperature = 37 degrees) and 0.37 h (pH 1, temperature = 37 degrees).