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The recognition of E. coli glutamine tRNA by glutaminyl-tRNA synthetase
M J Rogers1, I Weygand-Durasević, E Schwob
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
A variety of genetic, biochemical and structural studies have been used to determine factors ensuring the accuracy of recognition by aminoacyl-tRNA synthetases for tRNA. The identity elements of Escherichia coli tRNA(Gln) are located mainly in the anticodon and acceptor stem, and ensure the accurate recognition of the tRNA by glutaminyl-tRNA synthetase. We summarize a number of experimental techniques to define the accuracy of aminoacylation in vivo and in vitro.