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Development of second generation monoclonal antibodies recognising Lewisy/b antigen by anti-idiotypic immunisation
L G Durrant1, A Singhal, E Jacobs
1Cancer Research Campaign Laboratories, University of Nottingham, England.
Hybridoma
|December 1, 1993
Abstract:
Five new monoclonal antibodies (Mabs) recognising the Lewisy/b hapten of the IgG isotype have been produced following immunisation with rat anti-idiotypic antiserum to C14, an IgM Lewisy/b hapten Mab and boosting with C14gp200 antigen. They have the same fine specificity as the original Mab binding to a cell surface and secreted antigen preferentially expressed by colorectal tumour cells.