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Human myelin basic protein (MBP) epitopes recognized by mouse MBP-selected T cell lines from multiple sclerosis
Y K Chou1, R E Jones, D Bourdette
1Department of Neuroimmunology Research, Department of Veterans Affairs Medical Center, Portland, OR 97201.
Journal of Neuroimmunology
|January 1, 1994
Summary
Multiple sclerosis (MS) patient T cells recognize mouse myelin basic protein (MBP). This finding is crucial for understanding MS pathogenesis and developing mouse models for humanized severe combined immunodeficiency (SCID) chimeras.
Area of Science:
- Neuroimmunology
- Immunology
- Molecular Biology
Background:
- Multiple sclerosis (MS) is an autoimmune disease targeting the central nervous system.
- Understanding T cell responses to myelin antigens is key to MS pathogenesis.
- Experimental autoimmune encephalomyelitis (EAE) is a common mouse model for MS.
Purpose of the Study:
- To determine if T cells from MS patients can recognize mouse myelin basic protein (MBP).
- To assess the feasibility of using mouse MBP in severe combined immunodeficiency (SCID) mouse-human chimeras for MS research.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) from 11 MS patients were analyzed for in vitro proliferation.
- T cell lines were generated and selected using mouse MBP.
- Proliferation assays were performed using mouse MBP, human MBP, and synthetic MBP peptides.
- MHC restriction was analyzed for one T cell line.
Main Results:
- 55% of MS patients showed in vitro proliferation in response to mouse MBP.
- Four out of five T cell lines generated from MS patients recognized mouse MBP.
- All five T cell lines responded to at least one synthetic peptide of human MBP.
- One T cell line demonstrated MHC class II restricted proliferation.
Conclusions:
- T cells from MS patients can recognize epitopes on mouse MBP.
- These findings suggest cross-reactivity between human and mouse MBP epitopes.
- This recognition is a critical factor for developing SCID mouse-human chimeras for MS research.