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Related Experiment Videos

A role for lectin interactions during human neutrophil aggregation

Y P Rochon1, S I Simon, E B Lynam

  • 1University of New Mexico Cancer Center, Albuquerque 87131.

Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1994
PubMed
Summary

Carbohydrates inhibit neutrophil aggregation, suggesting a lectin-like mechanism involving L-selectin. This discovery highlights the role of specific sugars in immune cell adhesion and function.

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Plug flow cytometry.

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Area of Science:

  • Immunology
  • Cell Adhesion Biology

Background:

  • Neutrophil aggregation is crucial for immune responses and involves L-selectin and Mac-1.
  • The role of carbohydrate interactions in neutrophil aggregation remained unclear.

Purpose of the Study:

  • To investigate the potential role of carbohydrate interactions in neutrophil aggregation.
  • To determine if L-selectin mediates carbohydrate-dependent neutrophil adhesion.

Main Methods:

  • Utilized mono- and polysaccharides to test inhibition of neutrophil aggregation.
  • Employed two-color fluorescence flow cytometry with L-selectin-deficient neutrophils.
  • Assessed carbohydrate competition with monoclonal antibody binding to leukocyte adhesion proteins.

Main Results:

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  • Specific polysaccharides (dextran sulfate, fucoidan) and phosphorylated monosaccharides inhibited neutrophil aggregation.
  • Nonphosphorylated sugars and certain other polysaccharides did not inhibit aggregation.
  • Carbohydrates competed with antibody binding to leukocyte adhesion proteins, implicating a lectin-like mechanism.

Conclusions:

  • Neutrophil aggregation involves a lectin-like mechanism.
  • L-selectin is likely the molecule mediating carbohydrate-dependent adhesive events in neutrophil aggregation.