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Updated: Aug 17, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
Location of the streptomycin ribosomal binding site explains its pleiotropic effects on protein biosynthesis
1Centro de Biología Molecular Severo Ochoa Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Spain.
Abstract:
Photoaffinity-labeling experiments using three nitroguaiacol ether streptomycin derivatives with spacers of different lengths between the antibiotic and the photoreactive moiety (8, 12 and 17 A) allow us to: (1) unambiguously locate the boundaries of the antibiotic binding site; and (2) test the topographical consistency of the photolabeling results. The streptomycin binding site is located in the interface between the ribosomal subunits, close to proteins S5 in the 30 S and to L11 in the 50 S ribosomal subunits. This location explains most of the antibiotic's pleiotropic effects on protein biosynthesis, especially those related to the tRNA selection mechanism, and it also correlates with the location of the ribosomal components involved in the different streptomycin phenotypes.
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