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Updated: Aug 11, 2026
08:37
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Quantitation of RNA using the polymerase chain reaction
K P Foley1, M W Leonard, J D Engel
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500.
Trends in Genetics : TIG
|November 1, 1993
Summary
Reverse transcriptase and polymerase chain reaction (RT-PCR) enable sensitive RNA detection. New methods overcome RT-PCR
Area of Science:
- Molecular Biology
- Biochemistry
Background:
- Reverse transcriptase and polymerase chain reaction (RT-PCR) offer rapid and sensitive RNA detection.
- The high sensitivity of RT-PCR can be a limitation for quantitative applications due to variability.
- Minor changes in reaction conditions can significantly impact amplification results.
Purpose of the Study:
- To address the limitations of RT-PCR in quantitative applications.
- To present techniques for accurate RNA quantitation using RT-PCR.
Main Methods:
- Utilizing sequential reverse transcriptase and polymerase chain reaction.
- Implementing recently developed techniques for RNA analysis.
Main Results:
- Overcoming the challenge of variability in RT-PCR reactions.
- Enabling accurate quantitation of RNA through improved RT-PCR methods.
Conclusions:
- New techniques allow for precise RNA quantification via RT-PCR.
- These advancements enhance the utility of RT-PCR for quantitative studies.
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