Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steel factor and c-kit regulate cell-matrix adhesion

T Kinashi1, T A Springer

  • 1Center For Blood Research, Boston, MA 02115.

Blood
|February 15, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Targeting of High-Affinity LFA-1 Does Not Augment Costimulation Blockade in a Nonhuman Primate Renal Transplantation Model.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Linker regions and flexibility around the metalloprotease domain account for conformational activation of ADAMTS-13.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Biology and physics of von Willebrand factor concatamers.

Journal of thrombosis and haemostasis : JTH·2011
Same author

Synergy of interleukin-5 with interleukin-2 in the generation of lymphokine-activated killer-cells.

International journal of oncology·2011
Same author

Temperature control during laser vessel welding.

Applied optics·2010
Same author

Immunoprecipitation.

Current protocols in immunology·2008
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
Same journal

Is low a go for pediatric AML?

Blood·2026
See all related articles

Steel factor regulates cell adhesion by stimulating mast cells to bind fibronectin, a process dependent on the c-kit tyrosine kinase. This highlights steel factor's role in cell adhesion.

Area of Science:

  • Cell Biology
  • Hematology
  • Developmental Biology

Background:

  • The Steel (SI) and white spotting (W) loci encode key signaling molecules involved in cell development and migration.
  • Steel factor (c-kit ligand) and c-kit (tyrosine kinase receptor) are crucial for primordial germ cells, melanoblasts, and hematopoietic cells.
  • Cell adhesion molecules are hypothesized to play a critical role in these developmental processes.

Purpose of the Study:

  • To investigate the role of steel factor and c-kit in cell-extracellular matrix adhesion.
  • To utilize bone marrow-derived mast cells as a model system for studying adhesion mechanisms.

Main Methods:

  • Examined mast cell adhesion to fibronectin and vitronectin.
  • Utilized mast cells from c-kit mutant mice.

Related Experiment Videos

  • Assessed adhesion in response to steel factor, interleukin-3, and interleukin-4.
  • Investigated the role of integrin VLA-5 and c-kit tyrosine kinase activity.

    • Main Results:

    • Steel factor stimulates mast cell binding to fibronectin and vitronectin, unlike IL-3 and IL-4.
    • Adhesiveness activation is transient, occurs at low steel factor concentrations, and requires integrin VLA-5.
    • Mast cells from c-kit mutant mice adhere to fibronectin with PMA but not steel factor, indicating c-kit kinase activation is necessary.
    • C-kit kinase activity is not required for direct adhesion mediated by c-kit.

    Conclusions:

    • Regulation of cell adhesion is a significant biological function of steel factor.
    • Steel factor signaling through c-kit tyrosine kinase modulates mast cell adhesion to the extracellular matrix.
    • These findings provide insight into the molecular mechanisms governing cell migration and differentiation.