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Phenotypic conversions in renal development

D Herzlinger1, R Abramson, D Cohen

  • 1Department of Physiology and Biophysics, Cornell University Medical College, New York, NY 10021.

Journal of Cell Science. Supplement
|January 1, 1993
PubMed
Summary
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Kidney development involves ureteric bud epithelia transforming into mesenchymal nephron progenitors via epithelial-to-mesenchymal transition. This finding reveals a new pathway for renal morphogenesis and cell generation.

Area of Science:

  • Developmental biology
  • Renal biology
  • Cell biology

Background:

  • Kidney epithelia originate from ureteric bud and metanephric blastema.
  • Metanephric blastema mesenchymal cells are thought to derive from intermediate mesoderm.
  • The ureteric bud forms the renal collecting system through branching morphogenesis.

Purpose of the Study:

  • To investigate the origin of mesenchymal nephron progenitors in kidney development.
  • To test the hypothesis that metanephric blastema progenitors derive directly from intermediate mesoderm.
  • To elucidate the cellular mechanisms driving renal morphogenesis.

Main Methods:

  • Direct lineage analysis in an organ culture system.
  • Utilizing an immortalized embryonic renal mesenchymal cell line (RSTEM-1).

Related Experiment Videos

  • Observing phenotypic conversions in vitro.

    • Main Results:

    • Mesenchymal nephron progenitors are immediate descendants of ureteric bud epithelia.
    • Ureteric bud epithelia undergo epithelial-to-mesenchymal transition to form mesenchymal progenitors.
    • The RSTEM-1 cell line exhibits phenotypic conversions, modeling in vivo processes.

    Conclusions:

    • Renal morphogenesis involves ureteric bud epithelia generating mesenchymal nephron progenitors.
    • This process includes epithelial-to-mesenchymal and mesenchymal-to-epithelial transitions.
    • The RSTEM-1 cell line provides a model for studying epithelial phenotype regulation in kidney development.