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The B cell-specific transcription factor BSAP regulates B cell proliferation
Y Wakatsuki1, M F Neurath, E E Max
1Mucosal Immunity Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
The Journal of Experimental Medicine
|April 1, 1994
Summary
B cell-specific activator protein (BSAP) drives B cell proliferation. Suppressing BSAP reduces proliferation and Ig class switching, while increasing BSAP stimulates it, indicating BSAP is a key regulator.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- B cell-specific activator protein (BSAP) is a transcription factor crucial for B cell development.
- Its role in regulating B cell proliferation and function requires further elucidation.
Purpose of the Study:
- To investigate the impact of BSAP activity on B cell proliferation.
- To determine if BSAP acts as a rate-limiting factor in B cell proliferation and immunoglobulin class switching.
Main Methods:
- Assessed BSAP activity in B cells stimulated by mitogens, antigen receptor, and T cell signals.
- Utilized antisense oligonucleotides to suppress BSAP activity.
- Overexpressed BSAP in B cells and plasma cells to evaluate its effect on proliferation.
Main Results:
- BSAP activity increased upon B cell activation.
- Antisense-mediated BSAP suppression significantly reduced B cell proliferation.
- BSAP overexpression or de novo expression stimulated B cell proliferation.
- BSAP suppression downregulated interleukin 4-induced Ig class switching.
Conclusions:
- BSAP activity is essential for B cell proliferation and plays a rate-limiting role.
- BSAP influences immunoglobulin class switching, potentially through direct effects or secondary to proliferation changes.