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The structure of the O-specific polysaccharide of Salmonella arizonae O62
E V Vinogradov1, Y A Knirel, N K Kochetkov
1M.M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Carbohydrate Research
|February 3, 1994
Abstract:
The O-specific polysaccharide was liberated by mild acid hydrolysis of the lipopolysaccharide (LPS) isolated from S. arizonae O62 by phenol-water extraction. The branched hexasaccharide repeating-unit of the O-specific chain of the O62 LPS contained L-rhamnose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galacturonic acid in molar ratios of 4:1:1. On the basis of methylation analysis, 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY) and 1D NOE spectroscopy, the following structure for the repeating unit of the O-specific polysaccharide was established: [formula: see text]