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Related Experiment Videos

Control of lymphocyte integrin function: evidence for multiple contributing factors

D G Stupack1, C Shen, J A Wilkins

  • 1Rheumatic Disease Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.

Cellular Immunology
|April 15, 1994
PubMed
Summary
This summary is machine-generated.

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Stimuli activate alpha 2 beta 1 integrin on Jurkat cells for collagen binding. Different activation pathways exist, with some being sensitive to staurosporine and others not, suggesting multiple factors control lymphocyte adhesion.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Lymphocyte adhesion is crucial for cellular functions.
  • The alpha 2 beta 1 integrin complex acts as a collagen receptor on lymphocytes.
  • Jurkat T cell leukemia expresses alpha 2 beta 1 integrin in a latent state.

Purpose of the Study:

  • To investigate the activation of alpha 2 beta 1 integrin on Jurkat cells.
  • To compare the effects of different stimuli on integrin activation and collagen binding.
  • To understand the mechanisms regulating lymphocyte adhesion.

Main Methods:

  • Jurkat cells were treated with stimuli: antibody to alpha 2 chain (JBS2), antibody to beta 1 chain (JB1B), and phorbol ester (PMA).
  • The activation of alpha 2 beta 1 integrin-dependent Jurkat binding to collagen was measured.

Related Experiment Videos

  • Sensitivity of activation pathways to staurosporine was assessed.
  • Combinatorial effects of stimuli were evaluated.

    • Main Results:

    • JBS2, JB1B, and PMA all induced alpha 2 beta 1-dependent Jurkat cell binding to collagen.
    • PMA-induced activation was sensitive to staurosporine, while JBS2 and JB1B effects were not.
    • Combinations of stimuli at saturating concentrations showed additive effects on cell adhesion.

    Conclusions:

    • Multiple factors contribute to the full functionality of integrins and cellular adhesion.
    • Different stimuli activate alpha 2 beta 1 integrin through distinct pathways.
    • These findings offer a basis for controlling the adhesive potential of lymphoid cells.