Simple device for continuous measurement of fluorescent anions and cations in the rat kidney in situ

A simple device for fluorescence measurements on kidney surface in situ (FKS) is described. The device consists of (1) an illuminating unit, (2) a detector, (3) an evaluation unit. The device was developed for measurement of tissue content of anionic sulfofluorescein and cationic 4(4-dimethylaminostyryl)-N-methylpyridinium (ASP) during its secretion by proximal renal tubular cells.