Identification of serotype-specific and nonserotype-specific B-cell epitopes of coxsackie B virus using synthetic...

Coxsackie B viruses are thought to be involved in the induction of myocarditis. However, the diagnosis of acute infections by serology even today is practically impossible. The major problem is that no antigenic determinants of coxsackie B viruses have been identified or characterized which could be used as antigens in a rapid routine antibody screening test. Therefore, we synthesized overlapping peptides according to the sequence of the capsid protein VP1 of coxsackie B3 (CB3) virus in order to identify antigenic determinants located on VP1. Using sera raised against CB3 in mice, we were able to identify several antigenic determinants of CB3. Here we present a characterization of three epitopes found. We also tested the type-specificity of these antigenic determinants by using rabbit antisera against coxsackie viruses B1-B6. One antigenic determinant, peptide VP1-1, representing residues 1-15 of VP1, reacted highly type-specific for CB3. A second antigenic determinant (peptide VP1-3, residues 21-35 of VP1) reacted as well with the anti-CB3 sera as with the anti-CB4 sera. Therefore, the peptide VP1-3 seems to represent a non-type-specific antigenic determinant of coxsackie B viruses. Peptide VP1-24 (residues 229-243 of VP1) showed broad cross-reaction with anti-CB sera except with the anti-CB1 sera. This identification of type-specific and non-type-specific epitopes of coxsackie B viruses may provide the basis for the establishment of an effective and fast antibody screening test for coxsackie B viruses in patients with clinically suspected myocarditis.