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Related Experiment Videos

A growth factor phenotype map for ovine preimplantation development

A J Watson1, P H Watson, M Arcellana-Panlilio

  • 1Department of Obstetrics and Gynaecology, University of Western Ontario, London, Canada.

Biology of Reproduction
|April 1, 1994
PubMed
Summary

Growth factor gene expression is crucial for early sheep embryo development. Key genes like IGF and TGF are present, with potential oviduct-embryo signaling pathways identified.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Early embryonic development relies on complex molecular signaling.
  • Growth factors play vital roles in mammalian preimplantation development.
  • Understanding these factors in sheep is crucial for reproductive research.

Purpose of the Study:

  • To investigate the expression patterns of growth factor and receptor genes during ovine preimplantation development.
  • To explore potential paracrine interactions between the oviduct and early embryo.
  • To identify key molecular regulators of early sheep embryogenesis.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was employed.
  • Gene expression was analyzed in ovine embryos from 1-cell to blastocyst stages.

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  • Gene expression was also assessed in ovine oviduct cell cultures.

    • Main Results:

    • Transcripts for IGF-I, IGF-II, TGF alpha, and their receptors were detected throughout preimplantation development.
    • Basic fibroblast growth factor (bFGF) mRNA decreased in abundance from 1-cell to blastocyst stages.
    • Ovine trophoblast protein (oTP) transcripts were specific to blastocyst-stage embryos.
    • Oviduct cells expressed IGF-II, IGF-I, TGF alpha, bFGF, TGF beta 1, and insulin/IGF-I receptors, suggesting paracrine signaling.
    • Insulin, EGF, and NGF transcripts were notably absent in both embryos and oviduct cells.

    Conclusions:

    • Growth factor gene expression is evident early in ovine development, suggesting essential roles.
    • Paracrine signaling between the oviduct and embryo likely contributes to early development.
    • Further research is needed to elucidate the precise functions of these growth factor pathways in sheep embryogenesis.