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Related Experiment Videos

Potential-induced changes in intracellular pH

V Lyall, T U Biber

    The American Journal of Physiology
    |May 1, 1994
    PubMed
    Summary
    This summary is machine-generated.

    Intracellular pH (pHi) is linked to membrane potential (Vm) in many cells. This connection allows cells to adjust pHi in response to stimuli, potentially involving sodium channels in some tissues.

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    Area of Science:

    • Cellular Physiology
    • Biophysics

    Background:

    • Intracellular pH (pHi) is strongly dependent on membrane potential (Vm) across various cell types and tissues.
    • Changes in Vm, influenced by hormones and ion concentrations, suggest a mechanism for cells to modify pHi in response to environmental cues.
    • H+ flux is traditionally attributed to H+ channels sensitive to divalent metal ions.

    Purpose of the Study:

    • To investigate the role of amiloride-sensitive apical Na+ channels in H+ flux in Na(+)-transporting epithelia.
    • To explore the modulation of H+ flux via Na+ channels by hormones and second messengers.
    • To elucidate the significance of H(+)-conductive pathways in cellular signal transduction.

    Main Methods:

    • Analysis of H+ flux across cell membranes.
    • Investigation of ion channel activity, specifically amiloride-sensitive Na+ channels.

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  • Examination of the effects of hormones and intracellular second messengers on H+ flux.

    • Main Results:

    • A significant portion of H+ flux in Na(+)-transporting epithelia occurs through amiloride-sensitive apical Na+ channels, which are insensitive to divalent metal ions.
    • H+ flux via these Na+ channels is modulated by natriferic hormones and intracellular second messengers.
    • Potential-dependent changes in pHi are identified as a key cellular response mechanism.

    Conclusions:

    • Amiloride-sensitive Na+ channels play a crucial role in regulating H+ flux and intracellular pH in specific epithelial tissues.
    • H(+)-conductive pathways, including Na+ channels, are implicated in cellular signal transduction processes.
    • The interplay between membrane potential and intracellular pH offers a dynamic mechanism for cellular adaptation.