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Updated: Jan 12, 2026

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Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture
Published on: November 22, 2024
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[Incorporation of S-adenosylmethionine in the isolated and perfused rat liver. Preliminary study]
Bollettino Della Societa Italiana Di Biologia Sperimentale
|November 15, 1978
Abstract:
The uptake of labeled S-adenosylmethionine (SAM) by isolated and perfused rat liver has been compared to that of methionine: the rate of incorporation of the amino acid exceeds by about three times that of the sulfonium compound. S-Adenosylmethionine transport system shows saturation kinetics with an apparent Km of 59,5 microM and is energy-dependent, as demonstrated by the inhibition by 2,4-dinitrophenol.

