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Canine colonic circular muscle generates action potentials without the pacemaker component

L W Liu1, J D Huizinga

  • 1Department of Biomedical Science, McMaster University, Hamilton, ON, Canada.

Canadian Journal of Physiology and Pharmacology
|January 1, 1994
PubMed
Summary
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Canine circular muscle cells generate electrical oscillations, similar to spike-like action potentials, indicating an intrinsic smooth muscle cell property. This differs from the pacemaker activity of interstitial cells of Cajal in colonic slow waves.

Area of Science:

  • Gastroenterology
  • Physiology
  • Cell Biology

Background:

  • Canine colon exhibits slow wave and spike-like action potentials (SLAPs).
  • Slow waves originate from interstitial cells of Cajal (ICCs) and have a pacemaker component.
  • SLAPs are prominent in longitudinal muscle, but circular muscle contribution is unclear.

Purpose of the Study:

  • To investigate the intrinsic electrical activity of canine colonic circular muscle cells.
  • To determine if circular muscle cells can generate action potentials independently of ICCs and other muscle layers.

Main Methods:

  • Isolated canine colonic circular muscle preparations were used.
  • Electrical activity was recorded in quiescent preparations.
  • Barium (Ba2+) and Bay K8644 were used to induce and modulate electrical oscillations.

Related Experiment Videos

  • D600 and forskolin were used to investigate ion channel involvement and signaling pathways.

    • Main Results:

    • Circular muscle preparations were quiescent with a resting potential of -62.9 mV.
    • Ba2+ induced spontaneous electrical oscillations mimicking SLAPs, with a frequency of 6.6 cpm.
    • Bay K8644 increased oscillation frequency to 16.8 cpm.
    • These oscillations were abolished by D600, suggesting L-type calcium channel involvement.
    • Forskolin decreased oscillation amplitude but not frequency.

    Conclusions:

    • Canine circular muscle cells are intrinsically excitable and capable of generating oscillatory activity.
    • The generation of SLAPs appears to be an intrinsic property of smooth muscle cells.
    • The submuscular ICC-smooth muscle network is crucial for initiating the pacemaker component of colonic slow waves.
    • The mechanisms regulating slow wave frequency and circular muscle oscillations differ.