Related Experiment Video
Updated: Jul 25, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
Cross-linking of a sequential epitope within the beta-chain of HLA-DR/DP molecules suppressing B lymphocyte growth
A A Ghaderi1, G Gaudernack, D R Stanworth
1Department of Immunology and Microbiology, Medical School, Shiraz University of Medical Science, Iran.
Abstract:
A monoclonal antibody (SU2) directed against HLA class II antigens has been produced by immunizing BALB/c mice with a lymphoblastoid cell line, RPMI-8866 cells. The specificity of this mAb has been determined using a panel of HLA class II transfectants. SU2 stained all HLA-DR and HLA-DP transfectants tested, but reacted with only one HLA-DQ (HLA-DQw2). From comparison of the available data sequences of known HLA class II molecules, it appeared that the epitope recognized by the SU2 mAb contains a sequence of 6 amino acids (DSDVGE) at position 41-46 of the beta-chain of HLA-DR/DP. Functional studies indicated that SU2 mAb strongly induced cell aggregation and large clumping in resting tonsillar B cells. SU2 mAb inhibited the spontaneous growth and proliferation of B lymphoblastoid cell lines and drastically inhibited [3H]thymidine uptake by phorbol 12-myristate 13-acetate (PMA)-activated resting B cell. Results are discussed in relation to the dual recognition of HLA-DR/DP by SU2 mAb.
Related Concept Videos
Cell-mediated Immune Responses
Cross-reactivity
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

