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Related Experiment Videos

Apoptosis in human skin development: morphogenesis, periderm, and stem cells

R R Polakowska1, M Piacentini, R Bartlett

  • 1Department of Dermatology, University of Rochester, New York 14642.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 1, 1994
PubMed
Summary

Programmed cell death, or apoptosis, is crucial for shaping human skin development and forming epidermal appendages. This process is linked to cell differentiation and may involve specific survival genes like bcl-2.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Dermatology

Background:

  • Human skin development involves keratinocyte differentiation and stratification to form the epidermis.
  • Fetal skin basal cells give rise to epidermal appendages like hair follicles and glands.
  • The role of programmed cell death in epidermal architecture is often overlooked.

Purpose of the Study:

  • To investigate the function of programmed cell death in human skin development and epidermal architecture.
  • To explore the involvement of apoptosis in the remodeling of epidermal appendages.
  • To examine the expression patterns of bcl-2 in relation to cell death pathways and stem cell markers.

Main Methods:

  • Immunohistochemical localization of transglutaminases in fetal skin tissues.

Related Experiment Videos

  • Assessment of DNA fragmentation to indicate apoptosis.
  • Analysis of bcl-2 gene expression patterns.

    • Main Results:

    • Transglutaminase localization and DNA fragmentation indicate apoptosis in fetal periderm, intermediate epidermal cells, and appendages.
    • Apoptosis appears to be involved in the deletion of stage-specific cells and appendage remodeling.
    • Bcl-2 expression is distinct from cell death markers and correlates with specific morphogenetic events, suggesting a role as a stem cell marker in hair follicles.

    Conclusions:

    • Programmed cell death (apoptosis) plays a significant role in establishing the architecture of the human epidermis and its appendages.
    • Terminal differentiation of epidermal cells may represent a specialized form of apoptosis.
    • Bcl-2 protein may serve as a stem cell marker in specific regions of the hair follicle.