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Apolipoprotein E polymorphism in patients with acute pancreatitis
A Rollan1, G Loyola, C Covarrubias
1Department of Gastroenterology, Faculty of Medicine, Pontificia Universidad Católica, Santiago, Chile.
Pancreas
|May 1, 1994
Summary
Apolipoprotein E (Apo E) genetic variations do not explain abnormal chylomicron remnant clearance in acute pancreatitis (AP) patients. Further research into the LDL-related receptor protein (LRP) may reveal the cause.
Area of Science:
- Biochemistry
- Genetics
- Gastroenterology
Background:
- Patients with previous acute pancreatitis (AP) may exhibit abnormal catabolism of chylomicron remnants (CMR).
- Apolipoprotein E (Apo E) genetic polymorphism is known to influence CMR clearance.
- Investigating Apo E phenotypes in AP patients is crucial for understanding CMR metabolism.
Purpose of the Study:
- To compare the frequency distribution of Apo E phenotypes in patients with AP, gallstones, and control subjects.
- To determine if Apo E genetic polymorphism is associated with abnormal CMR catabolism in AP.
- To explore alternative explanations for impaired CMR clearance in AP.
Main Methods:
- Apo E phenotypes were analyzed using isoelectric focusing and immunoblotting.
- Study included 52 patients with AP, 109 with gallstones, and 110 controls.
- Fasting triglyceride levels were compared after adjusting for age and gender.
Main Results:
- No significant differences in Apo E phenotype frequency distribution were observed between the AP, gallstone, and control groups.
- The distribution of Apo E phenotypes was in Hardy-Weinberg equilibrium across all groups.
- Gene frequencies for Apo E2, E3, and E4 showed no statistically significant variations among the study groups.
Conclusions:
- Abnormal catabolism of CMR in AP patients is not attributable to Apo E polymorphism.
- The findings suggest that other factors, potentially involving the hepatocytic Apo E receptor (LRP), may be responsible for impaired CMR clearance in AP.