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Morphometric quantitation of stroma in human benign prostatic hyperplasia
R E Deering1, S A Bigler, J King
1Department of Urology, University of Washington, Seattle.
Urology
|July 1, 1994
Summary
Benign prostatic hyperplasia (BPH) is primarily composed of stroma, ranging from 50-75% of tissue. Stromal percentage is consistent across open prostatectomy, TURP, and needle biopsy samples, aiding BPH treatment selection.
Area of Science:
- Urology
- Pathology
- Medical Imaging
Background:
- Benign prostatic hyperplasia (BPH) involves stromal, epithelial, and luminal components.
- Accurate histologic quantitation of BPH may guide treatment decisions for bladder outlet obstruction.
Purpose of the Study:
- To investigate patient variations in the stromal percentage of BPH.
- To compare stromal percentage across different BPH tissue sampling methods.
Main Methods:
- Quantitative morphometry was performed on BPH specimens from open prostatectomy, transurethral resection of the prostate (TURP), and prostate needle biopsy (PNB).
- Specimens were stained for muscle-specific actin to identify stroma.
- Computer image analysis quantified stromal percentage.
Main Results:
- The stromal percentage in BPH ranged from 49.9% to 76.7% (mean 65.4%).
- No significant difference in stromal percentage was found between open prostatectomy, TURP, and PNB samples (p=0.70).
- A significant inverse relationship was observed between biopsy stromal percentage and smooth muscle content in PNB specimens (r²=0.49, p=0.021).
Conclusions:
- Stroma constitutes the majority of BPH tissue (approximately 50-75%).
- Stromal percentage is consistent across large (open prostatectomy) and small (PNB) tissue samples.
- Increased stromal percentage in biopsies may indicate higher nonmuscular stromal elements.