Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimal method for RNA extraction from mouse glomeruli

J J Baelde1, E C Bergijk, P J Hoedemaeker

  • 1Department of Pathology, University of Leiden, The Netherlands.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carnosinase Gene-Is It Responsible for Diabetic Nephropathy?: Carnosine as a Protective Factor in Diabetic Nephropathy: Association with a Leucine Repeat of the Carnosinase Gene CNDP1. Diabetes 54: 2320-2327, 2005.

Journal of the American Society of Nephrology : JASN·2023
Same author

Clinical outcomes in chronic intervillositis of unknown etiology.

Placenta·2020
Same author

Towards standardized criteria for diagnosing chronic intervillositis of unknown etiology: A systematic review.

Placenta·2017
Same author

Stability and Species Specificity of Renal VEGF-A Splicing Patterns in Kidney Disease.

PloS one·2016
Same author

The use of thyroid hormone in the treatment of depression: a review.

Acta neuropsychiatrica·2016
Same author

OS050. Genetic variants in pre-eclampsia: a meta-analysis.

Pregnancy hypertension·2015

This study presents the first optimal protocol for extracting RNA from mouse glomeruli, a crucial step for studying kidney diseases like lupus nephritis.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Background:

  • RNA extraction from mouse glomeruli is not well-established due to their small size.
  • Existing methods for rat and rabbit glomeruli are not suitable for mice.
  • Developing a reliable RNA extraction protocol is essential for research on mouse models of kidney disease.

Purpose of the Study:

  • To develop and optimize a protocol for extracting high-quality RNA from isolated mouse glomeruli.
  • To compare different RNA extraction methods and assess the impact of sample handling on RNA yield and integrity.
  • To validate the optimized protocol in an in-vivo model of experimental lupus nephritis.

Main Methods:

  • Mouse kidneys were perfused and glomeruli isolated using magnetic separation after sieving.

Related Experiment Videos

  • Comparative analysis of lithium chloride and caesium chloride RNA extraction methods.
  • Investigation of protease inhibitors and pre-isolation kidney freezing on RNA quality.

    • Main Results:

    • The lithium chloride method yielded higher RNA quantities and better integrity compared to the caesium chloride method.
    • Protease inhibitors and pre-isolation freezing negatively impacted RNA yield and quality.
    • The optimized protocol was successfully applied to RNA extraction from glomeruli in a lupus nephritis mouse model.

    Conclusions:

    • The lithium chloride method is optimal for RNA extraction from mouse glomeruli.
    • Proper sample handling, avoiding freezing, is critical for preserving RNA integrity.
    • This protocol facilitates molecular studies of kidney diseases in mouse models.