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Related Experiment Videos

Human kit ligand (stem cell factor) modulates platelet activation in vitro

J Grabarek1, J E Groopman, Y R Lyles

  • 1Department of Medicine, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215.

The Journal of Biological Chemistry
|August 26, 1994
PubMed
Summary

Human stem cell factor (SCF), or c-Kit ligand (KL), is present on platelets and can enhance platelet activation. This discovery suggests SCF/KL may play a role in hemostasis and conditions like atherosclerosis.

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Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Signaling

Background:

  • Human stem cell factor (SCF), also known as c-Kit ligand (KL), is a hematopoietic growth factor.
  • SCF/KL interacts with its receptor, c-Kit, to stimulate cell proliferation.

Purpose of the Study:

  • To investigate the presence and function of the c-Kit receptor on human platelets.
  • To determine if SCF/KL influences platelet activation and function.

Main Methods:

  • Polymerase chain reaction (PCR) to detect c-kit gene expression in platelets.
  • Radioligand binding assays using 125I-KL/SCF to demonstrate c-Kit receptor presence.
  • Immunoreactivity and protein characterization to confirm c-Kit protein identity.

Main Results:

Related Experiment Videos

  • The c-kit gene and c-Kit protein were identified on human platelets.
  • c-Kit on platelets is tyrosine-phosphorylated and associated with a phosphatidylinositol 3-kinase fragment.
  • SCF/KL augmented secondary platelet aggregation and serotonin secretion induced by epinephrine and ADP, partly via the thromboxane pathway.

Conclusions:

  • A novel platelet surface molecule, c-Kit, has been identified.
  • SCF/KL can modulate platelet activation, suggesting a role in hemostasis.
  • SCF/KL may be particularly relevant in conditions involving mesenchymal cells and circulating blood, such as wound healing and atherosclerosis.