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Two color flow cytometric analysis of concomitant acute myeloid leukemia and chronic lymphocytic leukemia

K R Tamul1, D C Meyers, S A Bentley

  • 1Clinical Microbiology-Immunology Laboratories, UNC Hospitals, Chapel Hill 27514.

Cytometry
|March 15, 1994
PubMed

During evaluation for macrocytic anemia and thrombocytopenia, a 74-year-old female was found to have a leukocytosis with two apparent populations of malignant cells identified in her peripheral blood smear and bone marrow aspirate. Morphologic characteristics of the two cell types were unusual, and cytochemical assays yielded ambiguous results. Two-color flow cytometric analysis demonstrated two distinct cell populations with immunophenotyping patterns consistent with chronic lymphocytic leukemia (CD5+/CD20+) and acute myelocytic leukemia (CD33+/CD34+), detected concurrently. The concomitant appearance of CLL and AML has been reported only rarely. The use of two-color flow cytometry to differentiate the populations demonstrates the utility of this technology in resolving unusual hematological malignancies.

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...