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Related Experiment Videos

Axon-glia interactions regulate ECM patterning in the postnatal rat olfactory bulb

M L Gonzalez1, J Silver

  • 1Department of Neurosciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4975.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|October 1, 1994
PubMed
Summary

The precise ring pattern of extracellular matrix (ECM) components like tenascin (TN) and chondroitin-6-sulfate proteoglycan (C-6S-PG) around olfactory bulb glomeruli depends on the presence of olfactory axons. Disruption of axons leads to disorganized ECM and cell patterns, which recover as axons regenerate.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Extracellular Matrix Biology

Background:

  • The olfactory bulb (OB) contains an extracellular matrix (ECM) including chondroitin-6-sulfate proteoglycan (C-6S-PG) and tenascin (TN).
  • This ECM is suggested to play a role in guiding olfactory axons to form glomeruli in the glomerular layer (GL).
  • A key question is whether the ring-like patterning of this ECM around glomeruli is determined by the matrix-producing cells or the developing axons.

Purpose of the Study:

  • To investigate the dependence of glial-associated matrix organization in the OB on the presence of olfactory axons.
  • To determine if the formation of TN/C-6S-PG ring patterns around glomeruli is a response to developing axons.

Main Methods:

  • Unilateral injection of a neurotoxin into the olfactory epithelium of postnatal rats to eliminate olfactory axons.

Related Experiment Videos

  • Assessment of glomerular number and organization using olfactory marker protein (OMP), beta-tubulin (TUJ1) antibodies, and Nissl staining.
  • Analysis of tenascin (TN), chondroitin-6-sulfate proteoglycan (C-6S-PG), and glial fibrillary acidic protein (GFAP) expression patterns.

    • Main Results:

    • Neurotoxin administration reduced glomeruli by 77.0%, with TN/C-6S-PG rings and periglomerular cells only surrounding remaining glomeruli.
    • Disruption of axons led to disorganized ECM and periglomerular cell configurations in the GL.
    • As olfactory axons regenerated and glomeruli reformed by 20 days, OMP, TUJ1, periglomerular cells, and TN/C-6S-PG rings reappeared in a glomerular-like pattern.

    Conclusions:

    • The formation of precise TN/C-6S-PG ring patterns around olfactory bulb glomeruli is dependent on the presence of bundled olfactory axons.
    • While initial gross positioning of glomeruli might involve ECM, the detailed patterning is an axonal response.
    • This suggests a dynamic interplay between axons and ECM in olfactory system development and organization.