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Expression pattern of parathyroid hormone/parathyroid hormone related peptide receptor mRNA in mouse postimplantation
M Karperien1, T B van Dijk, T Hoeijmakers
1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Abstract:
In this paper we describe the cloning of the mouse Parathyroid Hormone/Parathyroid Hormone related Peptide Receptor (PTH/PTHrPR) cDNA and expression of its mRNA during mouse postimplantation development from day 5.5 until day 15.5 post coitum (p.c.). In support of a model from previous studies, in which parietal endoderm differentiation is regulated by the interaction of the PTH/PTHrPR and Parathyroid Hormone related Peptide (PTHrP), high levels of PTH/PTHrPR mRNA levels were detected in developing parietal endoderm from day 5.5 p.c. and onwards. In the embryo proper, PTH/PTHrPR mRNA expression was mainly detected at sites of epithelium/mesenchyme interactions, starting at day 9.5 p.c. in the epithelium of the intestine and later in the mesenchyme of the lung, the epithelium of meso- and metanephric tubuli, the dermis and at all sites where bone formation takes place. The complexity of the PTH/PTHrPR expression pattern suggests tight developmental regulation and indicates multiple roles in embryogenesis for the receptor and its ligands, not only in extraembryonic tissue but also in the formation of various organs.
Insights
Researchers cloned the mouse Parathyroid Hormone/Parathyroid Hormone related Peptide Receptor (PTH/PTHrPR) and studied its mRNA expression during embryonic development. The receptor is crucial for parietal endoderm differentiation and organogenesis.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Parietal endoderm differentiation is thought to be regulated by the interaction between the Parathyroid Hormone/Parathyroid Hormone related Peptide Receptor (PTH/PTHrPR) and Parathyroid Hormone related Peptide (PTHrP).
- Understanding the expression patterns of PTH/PTHrPR is essential for elucidating its role in embryonic development.
Purpose of the Study:
- To clone the mouse PTH/PTHrPR cDNA.
- To analyze the spatiotemporal expression of PTH/PTHrPR mRNA during mouse postimplantation development (days 5.5–15.5 p.c.).
- To investigate the role of PTH/PTHrPR in embryonic development and organogenesis.
Main Methods:
- Cloning of the mouse PTH/PTHrPR cDNA.
- Quantitative analysis of mRNA expression using techniques like in situ hybridization or RT-PCR (specific method not detailed in abstract).
- Monitoring expression from day 5.5 to day 15.5 post coitum (p.c.).
Main Results:
- High levels of PTH/PTHrPR mRNA were detected in developing parietal endoderm from day 5.5 p.c. onwards, supporting its role in differentiation.
- In the embryo proper, PTH/PTHrPR mRNA expression was observed at sites of epithelium/mesenchyme interactions starting at day 9.5 p.c.
- Expression was noted in the intestine, lung mesenchyme, meso- and metanephric tubuli epithelia, dermis, and sites of bone formation.
Conclusions:
- The complex expression pattern of PTH/PTHrPR suggests intricate developmental regulation.
- The receptor and its ligands play multiple roles in embryogenesis, impacting both extraembryonic tissues and organ formation.
- PTH/PTHrPR is involved in parietal endoderm differentiation and various organ development processes.