Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Triton channels are sensitive to divalent cations and protons

T K Rostovtseva1, C L Bashford, A A Lev

  • 1Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, United Kingdom.

The Journal of Membrane Biology
|July 1, 1994
PubMed
Summary

Triton X-100 creates temporary aqueous pores in lipid bilayers, mimicking ion channels. These pores

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A study of sodium release in the course of ATP hydrolysis by membrane ATPase.

The Journal of membrane biology·2013
Same author

[Modeling of discrete currents of single ion channels of cell membranes using synthetic nanometer pores in polyethylene terephthalate films].

Tsitologiia·2008
Same author

The use of synthetic nanometer pores for modeling the conduction block of cation-selective channels of cell membranes by ruthenium red.

Doklady. Biochemistry and biophysics·2006
Same author

Modeling the effect of amiloride in studies of conductance of nanometer channels of synthetic track membranes.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2005
Same author

Conductance studies on trichotoxin_A50E and implications for channel structure.

Biophysical journal·2004
Same author

Understanding the tumor metabolic phenotype in the genomic era.

Current molecular medicine·2003

Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Physical Chemistry

Background:

  • Planar lipid bilayers are crucial models for studying cell membrane properties.
  • Ion channels regulate transport across membranes, but their formation mechanisms are complex.
  • Surfactants can alter lipid bilayer structure and function.

Purpose of the Study:

  • To investigate the formation of channel-like events in lipid bilayers induced by a non-ionic surfactant.
  • To determine the influence of ions and pH on these surfactant-induced events.
  • To explore the generalizability of ion sensitivity in lipidic pores.

Main Methods:

  • Formation of planar lipid bilayers using dioleoyl phosphatidyl choline, diphytanoyl phosphatidyl choline, or mono-oleoyl glycerol.
  • Addition of Triton X-100 to induce channel-like events.
  • Measurement of electrical conductivity across the bilayer.
  • Systematic addition and removal of divalent cations, protons, EDTA, and alkali to observe effects on conductivity.

Main Results:

  • Triton X-100 induced single channel-like events in various lipid bilayers.
  • Divalent cations and protons caused these events to disappear, an effect reversible by EDTA and alkali, respectively.
  • The conductance of remaining channels was not significantly affected by these ion/pH changes.

Conclusions:

  • Surfactant-induced aqueous pores in lipid bilayers exhibit ion and pH sensitivity.
  • This sensitivity is not exclusive to specific channel proteins or toxins.
  • Aqueous pores within a lipid environment can inherently possess ion- and pH-dependent gating properties.

Related Experiment Videos