Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Possible protective immunity in human opisthorchiasis

P S Akai1, S Pungpak, V Kitikoon

  • 1Department of Microbiology and Infectious Diseases, University of Calgary, Canada.

Parasite Immunology
|June 1, 1994
PubMed
Summary

Individuals in Thailand exposed to liver flukes Opisthorchis viverrini show higher antibody levels, suggesting potential immunological resistance to chronic infection. This research explores immune responses in those who remain uninfected despite exposure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adult Asthma Diagnosis: Physician Reported Challenges in Alberta-Based Primary Care Practices.

SAGE open nursing·2021
Same author

Structural and posttranslational analysis of human calcium-binding protein, spermatid-associated 1.

Journal of cellular biochemistry·2020
Same author

Stability of pro- and anti-inflammatory immune biomarkers for human cohort studies.

Journal of translational medicine·2017
Same author

The Canadian healthy infant longitudinal development birth cohort study: biological samples and biobanking.

Paediatric and perinatal epidemiology·2014
Same author

Mast cell differentiation and heterogeneity.

Immunology today·2014
Same author

Hepatitis E in a Canadian traveller.

The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012

Area of Science:

  • Parasitology
  • Immunology
  • Infectious Diseases

Background:

  • Chronic liver fluke infections (Opisthorchis viverrini, Clonorchis sinensis) impact over 30 million in Southeast Asia.
  • These infections cause significant morbidity, predisposition to cholangiocarcinoma, and frequent reinfection.
  • Heterogeneity in worm burden exists, with some exposed individuals remaining uninfected.

Purpose of the Study:

  • To investigate the immunological basis for resistance in individuals not shedding Opisthorchis viverrini eggs.
  • To compare antibody levels between infected and uninfected residents in an endemic area.

Main Methods:

  • Serum antibody levels (IgG, IgA, IgM) to O. viverrini were measured using enzyme-linked immunosorbent assays (ELISA).
  • Adult worm homogenate (AWH) and metacercaria homogenate (MH) were used as antigens.

Related Experiment Videos

  • Immunoblot analyses identified specific antigen reactivity in egg-negative individuals.

    • Main Results:

    • Egg-negative individuals exhibited significantly higher IgG, IgA, and IgM levels against AWH and total antibodies against MH compared to egg-positive individuals.
    • Egg-negative sera showed increased IgA reactivity to a 38-kDa AWH antigen.
    • Egg-negative sera demonstrated enhanced IgM reactivity to carbohydrate epitopes on a 42-kDa AWH glycoprotein.

    Conclusions:

    • Findings suggest that individuals remaining egg-negative may possess immunological resistance to Opisthorchis viverrini infection.
    • Specific antibody responses, particularly IgA and IgM, correlate with resistance.
    • This study provides evidence for immune mechanisms controlling chronic liver fluke infections.