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Growth factors and decidualization in vitro
J C Irwin1, L de las Fuentes, L C Giudice
1Department of Gynecology and Obstetrics, Stanford University Medical Center, California 94305.
Annals of the New York Academy of Sciences
|September 30, 1994
Summary
Insulin-like growth factors (IGFs) regulate decidualization, impacting prolactin and IGFBP-1 secretion in a dose-dependent manner. These findings highlight the IGF system
Area of Science:
- Reproductive Endocrinology
- Cellular Biology
- Growth Factor Signaling
Background:
- Growth factors are local regulators of endometrial cyclic activity.
- Limited information exists on growth factor regulation of decidual differentiation and function.
- Progesterone (P) induces characteristic changes in human endometrial stromal cells in vitro.
Purpose of the Study:
- To investigate the effects of insulin-like growth factors (IGF-I, IGF-II) on in vitro decidualization.
- To assess IGF regulation of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP-1) secretion.
- To explore the role of the IGF system in autocrine/paracrine interactions during decidualization.
Main Methods:
- Human endometrial stromal cells were cultured and treated with progesterone (P) and epidermal growth factor (EGF).
- Effects of IGF-I and IGF-II on cell proliferation, PRL, and IGFBP-1 secretion were measured.
- Dose-dependent responses and correlations between IGF-induced mitogenesis and IGFBP-1 levels were analyzed.
Main Results:
- IGFs, along with EGF and P, were required for stromal cell proliferation.
- IGFs regulated PRL and IGFBP-1 secretion in a dose-dependent biphasic manner, with peak stimulation at specific concentrations.
- High doses of IGFs inhibited IGFBP-1 secretion significantly while partially suppressing PRL secretion; IGF-induced mitogenesis inversely correlated with endogenous IGFBP-1.
Conclusions:
- Growth factor interactions, particularly the IGF system, play a crucial role in regulating decidual function.
- Specific cellular functions during decidualization are differentially regulated by growth factor interactions.
- The IGF system is implicated in autocrine/paracrine signaling during decidualization and early pregnancy, with IGF-II potentially involved in embryonic signaling.