Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adhesion molecules in hematopoietic cells

T Kinashi1, T A Springer

  • 1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.

Blood Cells
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Targeting of High-Affinity LFA-1 Does Not Augment Costimulation Blockade in a Nonhuman Primate Renal Transplantation Model.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Linker regions and flexibility around the metalloprotease domain account for conformational activation of ADAMTS-13.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Biology and physics of von Willebrand factor concatamers.

Journal of thrombosis and haemostasis : JTH·2011
Same author

Synergy of interleukin-5 with interleukin-2 in the generation of lymphokine-activated killer-cells.

International journal of oncology·2011
Same author

Temperature control during laser vessel welding.

Applied optics·2010
Same author

Immunoprecipitation.

Current protocols in immunology·2008
Same journal

In vitro and in vivo studies of stromal niches.

Blood cells·1994
Same journal

Differential manipulation of normal and chronic myeloid leukemia stem cell proliferation in vitro.

Blood cells·1994
Same journal

Enrichment for primitive hemopoietic progenitors of marrow cells from 5-fluorouracil-treated mice and normal mice.

Blood cells·1994
Same journal

Protein tyrosine phosphorylation in the regulation of hematopoiesis by receptors of the cytokine-receptor superfamily.

Blood cells·1994
Same journal

Lysis of blood platelets by human osteosarcoma cells in vitro.

Blood cells·1994
Same journal

Development of hemopoietic bone marrow within the ectopic bone induced by bone morphogenetic protein.

Blood cells·1994
See all related articles

Hematopoietic cell adhesion to stromal cells involves specific molecules like very late antigen-4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1). VLA-4 expression decreases during neutrophil differentiation, impacting cell adhesion.

Area of Science:

  • Hematology
  • Cell Biology
  • Immunology

Background:

  • Stromal cell interactions are vital for hematopoietic cell development.
  • Understanding adhesion molecules is key to characterizing these interactions.

Purpose of the Study:

  • To identify adhesion molecules mediating lymphoid, myeloid, and mast cell interactions with stromal cells.
  • To investigate the regulation of adhesion molecules during myeloid cell differentiation.

Main Methods:

  • Adhesion assays using various cell lines (lymphoid, myeloid, mast, myeloma) and stromal cells.
  • Analysis of adhesion molecule expression (VLA-4, VCAM-1, VLA-5, c-kit) and function.
  • Investigation of neutrophil differentiation using a precursor cell line (L-G3) and granulocyte colony-stimulating factor.

Related Experiment Videos

Main Results:

  • Very late antigen-4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) are critical for lymphoid and myeloid cell adhesion.
  • Myeloma cells adhere via hyaluronate.
  • Neutrophil differentiation induced by granulocyte colony-stimulating factor down-regulates VLA-4, affecting stromal cell adhesion.
  • Mast cells adhere to fibroblasts and stromal cells through VLA-5 (with steel factor) and c-kit (with membrane-anchored steel factor).

Conclusions:

  • Specific adhesion molecules mediate distinct hematopoietic cell interactions with stromal environments.
  • VLA-4 plays a significant role in myeloid cell adhesion and is regulated during differentiation.
  • Mast cell adhesion involves multiple pathways dependent on specific stimuli and receptors.