Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelets generated in vitro from proplatelet-displaying human megakaryocytes are functional

E S Choi1, J L Nichol, M M Hokom

  • 1AMGEN, Inc., Thousand Oaks, CA 91320.

Blood
|January 15, 1995
PubMed
Summary

This study introduces a novel in vitro system for generating functional platelets from human blood stem cells. The developed platelets mimic natural ones in structure and function, offering a new tool for research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unconventional bipartite entanglement in the quantum dimer magnet Yb<sub>2</sub>Be<sub>2</sub>SiO<sub>7</sub>.

Nature communications·2026
Same author

War and disaster are forcing a major rethink around mass casualty management.

BMJ military health·2023
Same author

Dynamical ground state in the XY pyrochlore Yb<sub>2</sub>GaSbO<sub>7</sub>.

npj quantum materials·2023
Same author

Survival of itinerant excitations and quantum spin state transitions in YbMgGaO<sub>4</sub> with chemical disorder.

Nature communications·2021
Same author

Magnetic ordering in the Ising antiferromagnetic pyrochlore Nd<sub>2</sub>ScNbO<sub>7</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2021
Same author

Possible itinerant excitations and quantum spin state transitions in the effective spin-1/2 triangular-lattice antiferromagnet Na<sub>2</sub>BaCo(PO<sub>4</sub>)<sub>2</sub>.

Nature communications·2020

Area of Science:

  • Hematology
  • Cell Biology
  • Biotechnology

Background:

  • Platelet production is crucial for hemostasis.
  • Current methods for studying platelet formation in vitro are limited.
  • Understanding megakaryocyte differentiation and platelet release is essential.

Purpose of the Study:

  • To develop and validate an in vitro culture system for sequential megakaryocyte development and platelet production.
  • To characterize the structural and functional properties of culture-derived platelets.
  • To establish a model for studying platelet formation and function.

Main Methods:

  • CD34-selected cells from human peripheral blood were cultured to promote megakaryocyte formation.
  • Mature megakaryocytes were replated to induce proplatelet development.

Related Experiment Videos

  • Proplatelets and derived platelets were analyzed for protein expression, ultrastructure, and function.
  • Platelet aggregation was assessed using agonists like thrombin and ADP, and inhibited with antibodies.

    • Main Results:

    • The culture system successfully generated mature megakaryocytes and proplatelets.
    • Proplatelets expressed key platelet-specific proteins (GPIb, GPIIb) and contained microtubule coils.
    • Culture-derived platelets were ultrastructurally identical to plasma-derived platelets.
    • These platelets aggregated in response to agonists and expressed activation markers (P-selectin).

    Conclusions:

    • A robust in vitro system for sequential megakaryocyte growth, development, and platelet production has been established.
    • The culture-derived platelets exhibit functional and structural characteristics of native platelets.
    • This system provides a valuable platform for investigating platelet biology and disorders.